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BSI BS ISO 10260:1992

Water quality. Measurement of biochemical parameters. Spectrometric determination of the chlorophyll-a concentration
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BSI BS ISO 10260:1992

Water quality. Measurement of biochemical parameters. Spectrometric determination of the chlorophyll-a concentration

PUBLISH DATE 2014
PAGES 16
BSI BS ISO 10260:1992

1.1 This International Standard specifies a method for the determination of the chlorophyll-a concentration. The procedure can be applied for phytoplankton in natural surface waters and for testing algal growth in bio-assays. Using appropriate sampling it can also be applied to phytobenthic communities (see annexĀ A).

1.2 Other algal pigments such as chlorophyll-b and chlorophyll-c and some chlorophyll metabolites do not contribute to the determination. Phaeopigments may be determined semiquantitatively, to correct for interference with chlorophyll-a determination and to indicate the portion of inactive algal biomass.

1.3 Chlorophyll is sensitive to light and oxygen, especially when it is extracted. To avoid oxidative and photochemical destruction, the samples shall not be exposed to bright light or air. Homogenization of the sample may in some cases increase the extraction efficiency.

1.4 The extraction procedure with ethanol involves heating to 75 °C for 5Ā min to inactivate chlorophyllase and accelerate the lysis of pigments. Storage of extracts (except filters containing suspended matter) prior to photometric measurement should be kept to a minimum, but is possible up to 3Ā d under refrigeration at 4 °C. Storage of extracts at less thanĀ āˆ’Ā 25 °C is possible for at least 30Ā d.

1.5 Even though the procedure involves filtration or centrifugation to clarify the final extract, a slight turbidity may remain. The acidification step may also cause turbidity. Therefore, the absorbance measured at 665Ā nm has to be corrected for turbidity by substracting the absorbance measured at 750Ā nm.

1.6 The pigment of certain rarely occurring phototrophic bacteria (e.g. Chlorobium) interferes with the determination of chlorophyll-a concentration [1]. The contribution of chlorophyll-b and chlorophyll-c to the absorbance at 665Ā nm is negligible [2].

SDO BSI: British Standards Institution
Document Number ISO 10260
Publication Date June 30, 2014
Language en - English
Page Count
Revision Level
Supercedes
Committee EH/3/2
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