Infant formula and adult nutritionals. Determination of <i>trans</i> and total (<i>cis</i> + <i>trans</i>) vitamin K<sub>1</sub> content. Normal phase HPLC
This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.
| SDO | BSI: British Standards Institution |
| Document Number | ISO 21446 |
| Publication Date | Jan. 9, 2019 |
| Language | en - English |
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| Committee | AW/34 |
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