ISO 19040-3:2018

Current Revision
Water quality - Determination of the estrogenic potential of water and waste water - Part 3: In vitro human cell-based reporter gene assay

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ISO 19040-3:2018

Water quality - Determination of the estrogenic potential of water and waste water - Part 3: In vitro human cell-based reporter gene assay

PUBLISH DATE 2018
ISO 19040-3:2018

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter gene assay is based on the activation of the human estrogen receptor alpha.

This method is applicable to:

  • fresh water;
  • waste water;
  • aqueous extracts and leachates;
  • eluates of sediments (fresh water);
  • pore water;
  • aqueous solutions of single substances or of chemical mixtures;
  • drinking water;

The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 0,3 ng/l and 1 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F).

The upper working range was evaluated [based on the results of the international interlaboratory trial (see Table F.3)] up to a level of 75 ng EEQ/l. Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification.

Extraction and pre concentration of water samples can prove necessary if their estrogenic potential is below the given LOQ.

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