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Biotechnology - Massively parallel sequencing - Part 3: General requirements and guidance for metagenomics

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Biotechnology - Massively parallel sequencing - Part 3: General requirements and guidance for metagenomics

PUBLISH DATE 2025

This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms.

The specified metagenomics process includes the following stages:

  • sampling strategy and process, including type, storage, transportation, extraction, quality;
  • nucleic acid library preparation
  • design and review process including sequencing strategy and assessment;
  • database construction;
  • bioinformatics analysis and report
  • validation and verification for bioinformatics pipeline, and database

This document applies to laboratories and research organizations.

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