Vegetable fats and oils - Determination of aflatoxins B1, B2, G1 and G2 by immunoaffinity column clean-up and high-performance liquid chromatography
This document specifies a method for the determination of the aflatoxins B1, B2, G1 and G2 in vegetable fats and oils, including peanut oil, sesame oil, olive oil, corn oil, sunflower oil, rapeseed oil and coconut oil, using immunoaffinity column clean-up and high-performance liquid chromatography with post-column derivatization.
The limits of quantification for the aflatoxins B1, B2, G1 and G2, and for the sum of aflatoxins B1, B2, G1 and G2, are 1Â g/kg, 0,25Â g/kg, 0,5Â g/kg, 0,25Â g/kg and 1Â g/kg, respectively.
The validation was carried out over the following concentration ranges:
—    aflatoxin B1 = 1 g/kg to 20 g/kg;
—    total aflatoxins = 2 g/kg to 52 g/kg.
| SDO | ISO: International Organization for Standardization |
| Document Number | ISO 20948 |
| Publication Date | Jan. 1, 2024 |
| Language | en - English |
| Page Count | 26 |
| Revision Level | |
| Supercedes | |
| Committee | ISO/TC 34/SC 11 |
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