Water quality - Detection of selected congeners of polychlorinated dibenzo-p-dioxins and polychlorinated biphenyls - Method using a flow immunosensor technique
This document specifies methods and principles for detection of selected congeners of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated biphenyls (PCBs) in water and wastewater using a flow immunosensor.
The flow immunosensor utilizes antibodies specific to 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) and 3,3,4,4,5-pentachlorobiphenyl (3,3,4,4,5-PeCB), which have the highest toxic equivalent factor (TEF) value among the congeners of each of PCDDs and PCBs.
The method is applicable to timely monitoring of selected congeners of 2,3,7,8-TCDD and 3,3,4,4,5-PeCB in water and wastewater to prioritize those for subsequent confirmatory determination.
This document specifies practical methods and procedures for sampling, extraction, clean-up, measurement in a flow immunosensor, data processing and validation of measurement results.
The combined use of automated instruments for extraction, clean-up, and flow immunosensing can reduce time-consumption and labour-intensity, while providing reproducible precise data.
This method can provide the lower limit of quantification (LOQ) for 2,3,7,8-TCDD and 3,3,4,4,5-PeCB of 28 pg/l and 152 pg/l, respectively at 20 % or less of coefficient variation (CV) depending on sampling, extraction, clean-up and measurement conditions.
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