ISO 29301:2023

Current Revision
Microbeam analysis - Analytical electron microscopy - Methods for calibrating image magnification by using reference materials with periodic structures

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ISO 29301:2023

Microbeam analysis - Analytical electron microscopy - Methods for calibrating image magnification by using reference materials with periodic structures

PUBLISH DATE 2023
ISO 29301:2023

This document specifies a calibration procedure applicable to images recorded over a wide magnification range in a transmission electron microscope (TEM).

The reference materials used for calibration possess a periodic structure, such as:

  • a diffraction grating replica,
  • a super-lattice structure of semiconductor,
  • an analysing crystal for X-ray analysis, and
  • a crystal lattice image of carbon, gold or silicon.

This document is applicable to the magnification of the TEM image recorded on a photographic film, or an imaging plate, or detected by an image sensor built into a digital camera.

This document also refers to the calibration of a scale bar.

This document does not apply to the dedicated critical dimension measurement TEM (CD-TEM) and the scanning transmission electron microscope (STEM).

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