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Animal feeding stuffs - Determination of available lysine
The method consists in hydrolysing with hydrochloric acid of a ground test portion, followed by separation of the total lysine by column chromatography and determination by spectrometry at 570 nm.
Reaction of a second ground test portion with an ethanolic solution of 2,4-dinitrofluorobenzene in an alkaline medium, purification, then hydrolysis with hydrochloric acid, separation of the non-available lysine by column chromatography, and determination by spectrometry at 570 nm.
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