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Animal feeding stuffs - Determination of free and total gossypol
The method is applicable to cottonseed and cottonseed meals and cakes.
The method consists in extracting of the compound in the presence of 3-aminopropane-1-ol, either with a mixture of 2-propanol and hexane for the determination of free gossypol or with dimethylformamide for the determination of total gossypol.
Conversion to gossypol-dianiline using aniline, and measurement of the absorbance at the wavelength of maximum absorbance (between 435 and 445 nm).
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