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Apple juice, apple juice concentrates and drinks containing apple juice -- Determination of patulin content -- Part 1: Method using high-performance liquid chromatography
The principle of the method specified is extraction of *patulin* from a test portion using ethyl acetate followed by partitioning of the extract with aqueous sodium carbonate solution.
Qualitative and quantitative determination of the patulin content is carried out by means of **high-performance liquid chromatography** using an ultraviolet detector.
The limit of detection is 10 µg/l, based on 5 ml of ready-to-drink apple juice.
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