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Apple juice, apple juice concentrates and drinks containing apple juice -- Determination of patulin content -- Part 2: Method using thin-layer chromatography
The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography.
The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution.
The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice.
The more precise method of analysis is HPLC.
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