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Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography
ISO 9936:2016 specifies a method for the determination of the contents of free α-, β-, γ-, and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).
For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.
Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this International Standard.
NOTE A suitable method involving a cold saponification procedure is described in Annex B for information only.
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