ISO 17099:2024

Current Revision
Radiological protection - Performance criteria for laboratories using the cytokinesis-block micronucleus (CBMN) assay in peripheral blood lymphocytes for biological dosimetry

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ISO 17099:2024

Radiological protection - Performance criteria for laboratories using the cytokinesis-block micronucleus (CBMN) assay in peripheral blood lymphocytes for biological dosimetry

PUBLISH DATE 2024
ISO 17099:2024

This document gives guidance on

  • confidentiality of personal information for the customer and the laboratory,
  • laboratory safety requirements,
  • calibration sources and calibration dose ranges useful for establishing the reference dose-response curves that contribute to the dose estimation from CBMN assay yields and the detection limit,
  • performance of blood collection, culturing, harvesting, and sample preparation for CBMN assay scoring,
  • scoring criteria,
  • conversion of micronucleus frequency in BNCs into an estimate of absorbed dose,
  • reporting of results,
  • quality assurance and quality control, and
  • informative annexes containing sample instructions for customers, sample questionnaire, a microscope scoring data sheet, and a sample report.

This document excludes methods for automated scoring of CBMN.

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